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KMID : 0881720210360020104
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Journal of Food Hygiene and Safety
2021 Volume.36 No. 2 p.104 ~ p.117
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Tetrodotoxin and Its Analogs: A Review of Analysis Methods and Levels in Pufferfish
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Park Bong-Ki
Jang Woo-Jin
Park Ki-Hwan
Lee Hee-Seok
Lee Wan-Ok
Choi Kwang-Sik
Lee Ji-Hyun
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Abstract
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Pufferfish is a major source of tetrodotoxin (TTX), a potent neurotoxin that can lead to death inhumans if consumed. The content of TTX and its analogs in different pufferfish species and the distribution in variousfish organs are poorly understood. In this study, we compared TTX analysis methods, including mouse bioassay,enzyme-linked immunosorbent assay, high-pressure liquid chromatography (HPLC) coupled with a fluorescencedetector, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry (LC-MS) methods.
Different HPLC columns (e.g., amide, hydrophilic interaction liquid chromatography, and C18 columns) and mobilephases were compared for TTX analysis using LC-MS. Additionally, published extraction methods were compared forLC-MS analysis. The content of TTX and its analogs in different pufferfish species and their distribution in variousorgans (e.g., liver, muscle, intestine, testis, ovary, and skin) were summarized. This information can help understandthe available TTX analysis methods, variation in TTX contents in pufferfish species from different regions, and TTXdistribution in various organs.
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KEYWORD
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Tetrodotoxin, Marine toxins, Neurotoxin, LC-MS/MS, Monitoring
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